Ultra Start 43 SERIES Manuel d'instructions

50105750-4 October 2010Thermo Fisher ScientificWX Ultra Series WX 100, WX 90, WX 80Instruction Manual

DescriptionGeneral description1-2 WX Ultra Series Thermo ScientificExternal view of ultracentrifugeThe WX Series ultracentrifuge have the same externa

OperationPrint utilities (option)2-84 WX Ultra Series Thermo ScientificContents of print utilitiesNo. Item Example printout1 CENTRIFUGE RUN RECORDS>

OperationPrint utilities (option)Thermo Scientific WX Ultra Series 2-853 RUN RECORDS OF A ROTOR>> Run records of a particular rotor are printed

OperationPrint utilities (option)2-86 WX Ultra Series Thermo ScientificAutomatic run result printingThis capability of the ultracentrifuge automatical

OperationPrint utilities (option)Thermo Scientific WX Ultra Series 2-871) Automatic printing of run recordsUltracentrifuge run records are retained i

OperationLockout (optional)2-88 WX Ultra Series Thermo ScientificLockout (optional)Logon1. Turn ON centrifuge2. Instead of a Run Screen, you will see

Thermo Scientific WX Ultra Series 3-13MaintenanceBefore conducting any maintenance, always read the following:Rotor chamberTo maintain the rotor chamb

MaintenanceCabinet3-2 WX Ultra Series Thermo ScientificDrive spindleWipe the drive spindle with a soft dry cloth every run to reduce the chance of the

MaintenanceService decontamination policyThermo Scientific WX Ultra Series 3-3instruction manual contains specific guidance about cleaning and decont

Thermo Scientific WX Ultra Series 4-14TroubleshootingBefore troubleshooting, always read the following:This ultracentrifuge incorporates a “self-diagn

Troubleshooting4-2 WX Ultra Series Thermo Scientific1. Searching for Alarm Information2. Responding to an alarm signalIf an alarm message appears, rem

DescriptionDesignThermo Scientific WX Ultra Series 1-3DesignControl panelThe control panel for the Sorvall WX Ultra series consists of a color screen

TroubleshootingDiagnosed problems - requiring maintenanceThermo Scientific WX Ultra Series 4-3Diagnosed problems - requiring maintenanceWhen a proble

Thermo Scientific WX Ultra Series 5-15PreinstallationThe information on these two pages describes the location requirements and the electrical service

PreinstallationElectrical requirements5-2 WX Ultra Series Thermo ScientificElectrical requirementsThe centrifuge must be connected to the power source

PreinstallationMoving the ultracentrifugeThermo Scientific WX Ultra Series 5-34. When the instrument is level, check that the four leveling screws ar

Thermo Scientific WX Ultra Series 6-16SpecificationsThe Sorvall WX Ultra series centrifuges are manufactured and tested according to the following reg

Specifications6-2 WX Ultra Series Thermo Scientific• EN 61000-3-3(*) EMC: Electromagnetic compatibilityThe Sorvall WX Ultra series centrifuges satisfy

Thermo Scientific WX Ultra Series 7-17Supply listThe items listed below are those supplied with the ultracentrifuge.Item name Part no. Q’ty Item drawi

Supply list7-2 WX Ultra Series Thermo ScientificOptional itemsItem name Part no. Q’ty Item drawing RemarksFunnel 45215 1Vinyl tube 45214 1Cleaning bar

Thermo Scientific WX Ultra Series A-1AService Decontamination PolicyIf a centrifuge or rotor that has been used with radioactive or pathogenic materia

Service Decontamination PolicyA-2 WX Ultra Series Thermo Scientificbe borne by the sender. Decontamination Information Certificates are included with

DescriptionDesign1-4 WX Ultra Series Thermo ScientificTable 1-1 [Functions of the display panel-keyed by item no. to fig. 1-2]No. Name and Symbol Func

Thermo Scientific WX Ultra Series B-1BCondensed operating instructionsSorvall® WX Ultra series ultracentrifugeThese instructions are intended to be us

Thermo Scientific RC 12BP+ C-1CChemical Compatibility Chart CHEMICALMATERIALALUMINUMANODIC COATING for ALUMINUMBUNA NCELLULOSE ACETATE BUTYRATEPOLYURE

C Chemical Compatibility ChartC-2 RC 12BP+ Thermo ScientificCesium BromideMSS- SSS- SSSSSSS- - SSSSSSMSSSCesium ChlorideMSSUSSS- SSSSSSS- - SSSSSSMSSS

C Chemical Compatibility ChartThermo Scientific RC 12BP+ C-3Hydrochloric Acid (conc.)UUUU- UUM- UMUUMUUU- S- USUUU- -Formaldehyde (40%)MMMSSSSMSSSSMS

C Chemical Compatibility ChartC-4 RC 12BP+ Thermo ScientificLactic Acid (20%)--SS-----MSM-SSSSSSSMSMSS- SN-Butyl AlcoholS- SU- - S- - SM- USMSSSSMMSM-

C Chemical Compatibility ChartThermo Scientific RC 12BP+ C-5Sucrose, AlkalineMSS- SSS- SSSSSSUSSSSSSSSMSSSSulfosalicylic AcidUUSSSSS- SSSUSSS- SSS- S

C Chemical Compatibility ChartC-6 RC 12BP+ Thermo ScientificChemical resistance data is included only as a guide to product use. Because no organized

Thermo Scientific D-1DWARRANTY THERMO FISHER SCIENTIFIC MAKES NO WARRANTY OF ANY KIND, EXPRESSED OR IMPLIED, INCLUDING THAT OF MERCHANTABILITY OR FITN

D WARRANTYD-2 Thermo Scientificc. The drive unit shall not be modified, disassembled, or repaired by any party but Thermo Fisher Scientific or by a se

Thermo Scientific E-1EContact InformationUnited States 866-9-THERMO+1 866 984 3766Canada +1 866 984 3766Austria +43 1 801 400Belgium +32 2 482 30 30Ge

DescriptionDesignThermo Scientific WX Ultra Series 1-5Table 1-2 [Functions of keyboard-keyed by item no. to fig. 1-3]No. Name and Symbol Functions an

E Contact InformationE-2 Thermo ScientificSpain +34 932 23 09 18Portugal +34 932 23 09 18Switzerland +41 44 454 12 12UK / Ireland +44 870 609 9203Chin

DescriptionDesign1-6 WX Ultra Series Thermo ScientificRotor chamberThe structure of the rotor chamber (vacuum chamber) is shown in Fig. 1-4.Figure 1-4

DescriptionDesignThermo Scientific WX Ultra Series 1-7Safety devices(1) ContainmentShould a rotor failure occur, the guard ring will contain the cont

DescriptionDesign1-8 WX Ultra Series Thermo ScientificRotor adapterOverspeed DecalThe overspeed decal located on the rotor base has alternating black

Thermo Scientific WX Ultra Series 2-12OperationThe Sorvall WX Ultra series ultracentrifuges are capable of operation in more than one mode to meet a w

OperationRun preparation2-2 WX Ultra Series Thermo ScientificRun preparationOptional featuresω2T settingCalculates and displays the running time from

OperationRun preparationThermo Scientific WX Ultra Series 2-3Starting up the ultracentrifugeBefore setting run conditions, display the run screen (sc

IMPORTANT! Please reference this page for the most up-to-date information on the following* • web site addresses • contact information • copyright

OperationBasic operation2-4 WX Ultra Series Thermo ScientificPreparing tubes/bottles and rotorThe Sorvall WX Ultra series ultracentrifuges allow you t

OperationBasic operationThermo Scientific WX Ultra Series 2-5[run screen]The screen for displaying run conditions and operational status is called th

OperationBasic operation2-6 WX Ultra Series Thermo ScientificFigure 2-4 Setting indicatorHow to set speed, running time, temperature, and other param

OperationBasic operationThermo Scientific WX Ultra Series 2-7Operation procedure1 Press cursor keys to enter the system into an input wait state.The

OperationBasic operation2-8 WX Ultra Series Thermo ScientificThe cursor blinks at one place.When one decimal place is not required, you do not need to

OperationBasic operationThermo Scientific WX Ultra Series 2-9Setting user ID codeThe USER ID CODE is a number to identify each user and can be set in

OperationBasic operation2-10 WX Ultra Series Thermo ScientificUsing Rotor Life ManagementThe rotor type and serial number may be set in rotor manageme

OperationBasic operating procedureThermo Scientific WX Ultra Series 2-11Basic operating procedureThere are two basic modes of operation, normal and z

OperationBasic operating procedure2-12 WX Ultra Series Thermo ScientificFigure 2-5 Basic operating procedureMove the cursor to ZONAL in the Run Scree

OperationBasic operating procedureThermo Scientific WX Ultra Series 2-13Normal operationGiven below is a description of the operational procedure for

Thermo Scientific WX Ultra Series iCChapter 0 Preface - Intended use and safety definitions ...

OperationBasic operating procedure2-14 WX Ultra Series Thermo ScientificThe run mode indicator on the display panel displays the following:Figure 2-7

OperationBasic operating procedureThermo Scientific WX Ultra Series 2-15Zonal operationZonal operation is a mode of operation using a zonal rotor or

OperationBasic operating procedure2-16 WX Ultra Series Thermo Scientific2 On the Run Screen, move the cursor to ZONAL and enter the following by opera

OperationAcceleration and deceleration ratesThermo Scientific WX Ultra Series 2-17Acceleration and deceleration ratesIn order to meet various experim

OperationAcceleration and deceleration rates2-18 WX Ultra Series Thermo Scientific* Minimum time is the time for accelerating or decelerating by the d

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-19Figure 2-9 Operation with setting ACCEL = 0 and DECEL = 0How to use the

OperationHow to use the FUNCTION field2-20 WX Ultra Series Thermo ScientificThe above features can be used in combination.When all settings are entere

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-21Programmed operationThis ultracentrifuge has a programmed operation featu

OperationHow to use the FUNCTION field2-22 WX Ultra Series Thermo ScientificBasic operation of the programmed operation featureUse cursor keys tosele

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-23Note You cannot create, change, or delete a program while in running. Pe

Contentsii WX Ultra Series Thermo ScientificCentrifuge scheduler...

OperationHow to use the FUNCTION field2-24 WX Ultra Series Thermo Scientific1. Programming procedure for run conditions (creating or changing)Shown be

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-255Enter a desired condition.For example, select “SPEED”.6 Select a desired

OperationHow to use the FUNCTION field2-26 WX Ultra Series Thermo Scientific6 To select a desired rotor from the “Rotor Management”:(1') Select “

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-27To change a run condition, use cursor keys to move to the item you want t

OperationHow to use the FUNCTION field2-28 WX Ultra Series Thermo Scientific10 Add Remark to the run conditions in the memory.(1) Press the ESC key.(2

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-2911 When the storing is over, press ESC key several times.>> The fir

OperationHow to use the FUNCTION field2-30 WX Ultra Series Thermo Scientific2. How to perform a programmed operationShown below is how to perform a “p

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-31(b) If you do not know which memory number you needStep Key operation Scr

OperationHow to use the FUNCTION field2-32 WX Ultra Series Thermo ScientificStep-mode operationThis ultracentrifuge incorporates a step-mode operation

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-33Figure 2-13 A typical step-mode runRun timeStep 1Step 2Step 3Speed (rpm)

Thermo Scientific WX Ultra Series iiiPPreface - Intended use and safety definitionsThis manual is a guide to the use of the Sorvall WX Ultra series ul

OperationHow to use the FUNCTION field2-34 WX Ultra Series Thermo ScientificStep Key operation Screen display and considerations1 While in the Run Scr

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-354 Select “Creating or Changing.”5Enter a desired condition.To select SPEE

OperationHow to use the FUNCTION field2-36 WX Ultra Series Thermo Scientific8 Lastly, specify run conditions for step 3.9Press the downward cursor key

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-3711 Add Remark to the run conditions in the memory.(1) Press the ESC key.(

OperationHow to use the FUNCTION field2-38 WX Ultra Series Thermo Scientific1. Step displayThe PROGRAM field displays steps as follows:When the system

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-39(2) Deleting a programThis section explains how to delete a set of run co

OperationHow to use the FUNCTION field2-40 WX Ultra Series Thermo Scientific(3) Other procedures1. Making changes to the run conditionsCall the memory

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-414. Control temperature: 4 °C5. Acceleration mode: 96. Deceleration mode:

OperationHow to use the FUNCTION field2-42 WX Ultra Series Thermo Scientific1. How to perform an RTC runStep Key operation Screen display and consider

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-434 Use cursor keys and numeric keys to enter month, day, hours, and minute

Preface - Intended use and safety definitionsImportant safety reminderiv WX Ultra Series Thermo ScientificImportant safety reminderCertain potentially

OperationHow to use the FUNCTION field2-44 WX Ultra Series Thermo Scientific1. For an RTC run, the run mode indicator on the display panel displays th

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-45Making changes to the RTC settingsThis section shows how to cancel or mak

OperationHow to use the FUNCTION field2-46 WX Ultra Series Thermo ScientificCanceling the RTC settingsThe canceling procedure varies depending on whet

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-472 Press cursor keys to blink RTC and then press ENTER key.3 [Before start

OperationHow to use the FUNCTION field2-48 WX Ultra Series Thermo ScientificDisplaying and setting RCFThis ultracentrifuge stores the maximum and aver

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-49Step Key operation Screen display and considerations1 While in the Run Sc

OperationHow to use the FUNCTION field2-50 WX Ultra Series Thermo Scientific• When the rotor to be used is not identified (when rotor type is displaye

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-514 Select a desired rotor. To select a desired rotor from the “Used Rotor

OperationHow to use the FUNCTION field2-52 WX Ultra Series Thermo Scientific4 To select a desired rotor from the “Rotor Management”: (1') Select

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-53How to set an RCF valueEnter a rotor number and an RCF value, and the mac

Preface - Intended use and safety definitionsImportant safety reminderThermo Scientific WX Ultra Series vWARNING• The use of sealed rotors, buckets a

OperationHow to use the FUNCTION field2-54 WX Ultra Series Thermo Scientific4 Select a desired rotor.To select a desired rotor from the “Used Rotor Li

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-554 To select a desired rotor from the “Rotor Management”:(1) Select “Rotor

OperationHow to use the FUNCTION field2-56 WX Ultra Series Thermo ScientificSetting ω2TThis machine is provided with a function to perform an ω2T run.

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-57Step Key operation Screen display and considerations1 Set the Speed in th

OperationHow to use the FUNCTION field2-58 WX Ultra Series Thermo ScientificDefrost functionIf the chamber door is left open between low temperature r

OperationHow to use the FUNCTION fieldThermo Scientific WX Ultra Series 2-59Setting and resetting zonal operationSelection between normal operation a

OperationRotor management2-60 WX Ultra Series Thermo Scientific2. Resetting zonal operationRotor managementIt is very important to manage the life of

OperationRotor managementThermo Scientific WX Ultra Series 2-61To utilize this feature, each time a rotor is used it must be recorded by the ultracen

OperationRotor management2-62 WX Ultra Series Thermo ScientificStep Key operation Screen display and considerations1 While in the Run Screen, use the

OperationRotor managementThermo Scientific WX Ultra Series 2-635 Select “T-890” rotor.6 After checking the specifications of the rotor, enter “Yes”.7

Preface - Intended use and safety definitionsImportant safety remindervi WX Ultra Series Thermo Scientific

OperationRotor management2-64 WX Ultra Series Thermo ScientificThus the rotor has been registered.Deleting a registered rotorIf a registered rotor bec

OperationRotor managementThermo Scientific WX Ultra Series 2-65Updating the existing data for a rotorWhen a rotor is registered in the Sorvall WX Ult

OperationWhen power failure occurs2-66 WX Ultra Series Thermo ScientificThus, the current total number of runs and hours have been updated.When power

OperationWhen power failure occursThermo Scientific WX Ultra Series 2-67Figure 2-16 Removing the front cover(4) Open the air vent (by turning the vac

OperationWhen power failure occurs2-68 WX Ultra Series Thermo ScientificFigure 2-17 Ultracentrifuge with the front cover removedFigure 2-18 Installing

OperationFeatures of the menu screenThermo Scientific WX Ultra Series 2-69Features of the menu screenPress MENU key on the key board, and the Menu Sc

OperationFeatures of the menu screen2-70 WX Ultra Series Thermo Scientific2. Deleting the centrifuge reservationYou can cancel the operation schedule

OperationFeatures of the menu screenThermo Scientific WX Ultra Series 2-71User listThe Centrifuge User List screen is displayed. This screen allows y

OperationFeatures of the menu screen2-72 WX Ultra Series Thermo Scientific• ID code and user name• Using example of ID code and user nameIf ID Code is

OperationFeatures of the menu screenThermo Scientific WX Ultra Series 2-733 Select “Creating”.4 Use cursor keys to highlight a charac-ter and press t

Thermo Scientific WX Ultra Series 1-11DescriptionGeneral descriptionThe Sorvall WX Ultra series ultracentrifuges are designed and manufactured based o

OperationFeatures of the menu screen2-74 WX Ultra Series Thermo Scientific2. Change of User NameNote Changing a User Name is limited to the person (w

OperationFeatures of the menu screenThermo Scientific WX Ultra Series 2-753. Deleting a user name setting6 After entering characters, use cursor keys

OperationFeatures of the menu screen2-76 WX Ultra Series Thermo ScientificAlarm informationThe Alarm Information screen displays the contents of the a

OperationFeatures of the menu screenThermo Scientific WX Ultra Series 2-77Rotor catalogYou can refer to rotor types and rotor specifications that are

OperationFeatures of the menu screen2-78 WX Ultra Series Thermo ScientificFigure 2-21 User Customizations screenFigure 2-22 Screen Utilities screen2.

OperationFeatures of the menu screenThermo Scientific WX Ultra Series 2-79Figure 2-24 Zoom screen3. Changing date and timeUse this option to precisel

OperationFeatures of the menu screen2-80 WX Ultra Series Thermo Scientific(2) Run Time rangeThis function selects one of the following two items as th

OperationPrint utilities (option)Thermo Scientific WX Ultra Series 2-81(4) Zonal speedThis function can change (in units of 100 rpm) the zonal speed

OperationPrint utilities (option)2-82 WX Ultra Series Thermo ScientificFigure 2-31 PRINT UTILITIES ScreenOperating procedureNote • Select “1. Printer

OperationPrint utilities (option)Thermo Scientific WX Ultra Series 2-837 Print program (1) On the Print Utilities menu screen, select “7. Print Progr